Skip to main content
. Author manuscript; available in PMC: 2018 Mar 24.
Published in final edited form as: J Mol Biol. 2017 Feb 1;429(6):790–807. doi: 10.1016/j.jmb.2017.01.021

Table 5.

Apparent second-order rate constants determined using CD for [2Fe-2S] cluster transfer to and from G208C NFU1. Native transfer rates were previously determined using the same CD method [1, 10, 14].

Rate Constant for Native
NFU1 (M−1 min−1)		 Rate Constant for G208C
NFU1 (M−1min−1)
Human NFU1 to human Fdx1 4700 ± 800 [1] 2600 ± 300
Human NFU1 to human Fdx2 3900 ± 1200 [1] 1200 ± 200
Human NFU1 to human Grx2 3740 ± 77 [10] 22400 ± 5000
Human NFU1 to S. cerevisiae Grx3 36200 ± 7700 [14] 14500 ± 3500
S. pombe Isa1 to human NFU1 6700 ± 1560 [10] No transfer
[2Fe-2S](GS)4 to human NFU1 1930 ± 210 [1] No transfer
Human IscU to human NFU1 4750 ± 8 [14] No transfer
Human IscU to human NFU1 with HSPA9/Hsc20 and MgATP No transfer No transfer
Human NFU1 to GSH to form [2Fe- 2S](GS)4 complex 130 ± 22 [1] 140 ± 20