Table 2. Primers and probes used for real time PCR.
Primer or probe | Nucleotide sequence | Working stock conc (μM) |
---|---|---|
F373 | ACGCAATCTAGCAGA TGAAGCA |
2.5 |
R424 | TCGTGCGTTTTAATTC CAGCT TGCCGAAAACGC”T”T |
2.5 |
Pb400i | GATACAGGGAG 5`Cy5;3`SpC6;”T”BHQ2 |
2.5 |