FIG. 2.

Effect of l-alanine on germination of spores of the wild-type strain and of mutant strains LH104 (gerRB) and LH129 (gerRC). Germination was monitored as the fall in OD₆₀₀ in response to l-alanine over the course of 60 min at 30°C and pH 7.4. ▪, wild-type strain, 100 mM l-alanine; □, wild-type strain, 10 mM l-alanine; ▴, wild-type strain, 1 mM l-alanine; ▵, wild-type strain, 0.1 mM l-alanine; ♦, wild-type strain, no germinant; •, LH104 (gerRB), 100 mM l-alanine; ○, LH129 (gerRC), 100 mM l-alanine. The data are means of values from duplicate experiments which were carried out with two independent spore batches.