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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Anaerobe. 2017 Mar 6;45:86–100. doi: 10.1016/j.anaerobe.2017.03.004

Figure 6. Effect of iLCA on TCA mediated spore germination and outgrowth, growth kinetics, and toxin activity of C. difficile strains.

Figure 6

(A) In vitro assay to assess if the addition of iLCA (0.01% and 0.1%) will inhibit TCA-mediated spore germination and outgrowth compared to positive controls, 0.1% TCA with ethanol (+TCA, red bar). All treatment groups were supplemented with 0.1% TCA. (B) Growth curves of C. difficile strains in BHI media with ethanol alone (solid lines), and the addition of 0. 0003% iLCA (dotted lines). The data presented represents OD600 ± SEM for positive controls and OD600 mean for iLCA from triplicate experiments. (C) Culture supernatants after 24 hr growth were used for a Vero cell cytotoxicity assay and the data is expressed as log10 reciprocal dilution toxin per 100 μ1 of C. difficile culture supernatant. C. difficile strains grown in BHI media with ethanol without SBAs (EthOH, positive control) and with iLCA (0.0003% and 0.00003%).