TABLE 3.
Simulation results for varying prevalence and contamination level on seeds with and without disinfection, where the detection limit for both pre- and post-production sampling was 2 log10CFU/g
Contamination level, disinfection status | Prevalence (% of positive 25-g samples) | % of samples in which contamination was detected
|
% of batches contaminated | % of batches reaching the consumer | |
---|---|---|---|---|---|
Preproductiona | Postproductionb | ||||
High (100 to 5,000 CFU/sample), without disinfection | 0.0001 | 0 | 13.7 | 13.7 | 0.0 |
0.001 | 0 | 80.9 | 80.9 | 0.0 | |
0.01 | 0.4 | 100.0 | 100.0 | 0.0 | |
0.1 | 39.6 | 100.0 | 100.0 | 0.0 | |
1 | 100.0 | 100.0 | 100.0 | 0.0 | |
Low (1 to 5 CFU/sample), without disinfection | 0.0001 | 0 | 12.1 | 13.7 | 1.6 |
0.001 | 0 | 66.2 | 74.6 | 8.4 | |
0.01 | 0.5 | 98.2 | 100.0 | 1.8 | |
0.1 | 35.9 | 99.9 | 100.0 | 0.1 | |
1 | 100.0 | 100.0 | 100.0 | 0.0 | |
High (100 to 5,000 CFU/sample), with disinfection (triangular log10 CFU reduction [1, 2.5, 6.5]) | 0.0001 | 0 | 6.4 | 8.0 | 1.6 |
0.001 | 0 | 45.4 | 49.7 | 4.3 | |
0.01 | 0.2 | 75.3 | 79.3 | 4.0 | |
0.1 | 24.1 | 83.7 | 85.2 | 1.5 | |
1 | 82.3 | 87.1 | 87.7 | 0.6 | |
Low (1 to 5 CFU/sample), with disinfection (triangular log10 CFU reduction [1, 2.5, 6.5]) | 0.0001 | 0 | 0.1 | 0.1 | 0.0 |
0.001 | 0 | 1.3 | 1.7 | 0.4 | |
0.01 | 0 | 4.2 | 5.0 | 0.8 | |
0.1 | 0.6 | 8.9 | 9.8 | 0.9 | |
1 | 6.3 | 13.7 | 14.7 | 1.0 |
Irrigation water from composite samples (50 subsamples of 25 g each) tested after 3 days of sprouting but before the entire batch of 36.3 kg of seeds was placed into production.
Irrigation water from 36.3 kg of the batch tested after 3 days of sprouting.