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. 2005 Feb;71(2):746–753. doi: 10.1128/AEM.71.2.746-753.2005

TABLE 3.

Simulation results for varying prevalence and contamination level on seeds with and without disinfection, where the detection limit for both pre- and post-production sampling was 2 log10CFU/g

Contamination level, disinfection status Prevalence (% of positive 25-g samples) % of samples in which contamination was detected
% of batches contaminated % of batches reaching the consumer
Preproductiona Postproductionb
High (100 to 5,000 CFU/sample), without disinfection 0.0001 0 13.7 13.7 0.0
0.001 0 80.9 80.9 0.0
0.01 0.4 100.0 100.0 0.0
0.1 39.6 100.0 100.0 0.0
1 100.0 100.0 100.0 0.0
Low (1 to 5 CFU/sample), without disinfection 0.0001 0 12.1 13.7 1.6
0.001 0 66.2 74.6 8.4
0.01 0.5 98.2 100.0 1.8
0.1 35.9 99.9 100.0 0.1
1 100.0 100.0 100.0 0.0
High (100 to 5,000 CFU/sample), with disinfection (triangular log10 CFU reduction [1, 2.5, 6.5]) 0.0001 0 6.4 8.0 1.6
0.001 0 45.4 49.7 4.3
0.01 0.2 75.3 79.3 4.0
0.1 24.1 83.7 85.2 1.5
1 82.3 87.1 87.7 0.6
Low (1 to 5 CFU/sample), with disinfection (triangular log10 CFU reduction [1, 2.5, 6.5]) 0.0001 0 0.1 0.1 0.0
0.001 0 1.3 1.7 0.4
0.01 0 4.2 5.0 0.8
0.1 0.6 8.9 9.8 0.9
1 6.3 13.7 14.7 1.0
a

Irrigation water from composite samples (50 subsamples of 25 g each) tested after 3 days of sprouting but before the entire batch of 36.3 kg of seeds was placed into production.

b

Irrigation water from 36.3 kg of the batch tested after 3 days of sprouting.