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. 2005 Feb;71(2):898–903. doi: 10.1128/AEM.71.2.898-903.2005

FIG. 1.

FIG. 1.

Nested PCR for the Cpgp40/15 gene and the SSU rRNA. (A) Nested PCR with templates of four kinds of parasites. Nested PCRs for the SSU rRNA gene and the Cpgp40/15 gene are shown in lanes 1 to 4 and 5 to 8, respectively. Lanes 1 and 5, C. muris RN66 strain; lanes 2 and 6, C. parvum Iowa strain; lanes 3 and 7, G. lamblia H3 strain; lanes 4 and 8, G. muris Roberts-Thompson strain. (B) Nested PCR with templates of C. parvum and C. hominis strains. Nested PCRs for the Cpgp40/15 and SSU rRNA are shown in lanes 1 to 3 and 4 to 6, respectively. Lanes 1 and 4, C. parvum Iowa strain; lanes 2 and 5, C. parvum HNJ-1 strain; lanes 3 and 6, C. hominis Ogose strain. Lanes M and N show DNA size standards (100-bp DNA ladder; Takara Shuzo) and the PCR negative control, respectively.