TABLE 2.
Recovery and detection of C. parvum by IMS-PCR and IMS-FA from oocyst-inoculated turbid water samples
| Sample type | Trial | Detection by nested PCR and FA at inoculation dose:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 600 oocysts
|
200 oocysts
|
100 oocysts
|
50 oocysts
|
||||||
| Detection | No. (%) | Detection | No. (%) | Detection | No. (%) | Detection | No. (%) | ||
| Low turbidity (200 NTU) | First | +++ | 312 (52%) | +++ | 76 (38%) | ++ | 58 (58%) | − | 17 (34%) |
| Second | +++ | 318 (53%) | − | 125 (63%) | − | 47 (47%) | − | 18 (36%) | |
| Third | NT | +++ | 117 (59%) | + | 67 (67%) | NT | |||
| Avg | 315 (53%) | 106 (53%) | 57 (57%) | 18 (36%) | |||||
| High turbidity (1,000 NTU) | First | ++ | 89 (15%) | + | 38 (19%) | − | 30 (30%) | − | 13 (26%) |
| Second | − | 134 (22%) | − | 39 (20%) | − | 35 (35%) | − | 15 (30%) | |
| Third | NT | − | 62 (31%) | − | 19 (19%) | NT | |||
| Avg | 112 (19%) | 46 (23%) | 28 (28%) | 14 (28%) | |||||
a
Detection: +++, detection in all three reaction systems; ++, detection in two reaction systems; +, detection in one reaction system; −, no detection. No. (%): number of oocysts confirmed by FA (% recovery). NT, not tested.