Table 2.
Gene-specific primers of lipid metabolism-related enzymes.
| Gene name1 | Primer sequence (5→3) | Tm (°C)/size (bp) | Accession |
|---|---|---|---|
| SREBP-1c | F:GAGGAAGGCCATCGAGTACA R:GGAAGACAAAGGCACAGAGG |
60.3/220 | AY029224 |
| FAS | F:CTATCGACACAGCCTGCTCCT R:CAGAATGTTGACCCCTCCTACC |
62.0/107 | J03860 |
| SCD1 | F:ACCATACATTCCCCTACGACT R:TTTTCCGGGCCAAGATGACC |
56.0/144 | NM204890 |
| ACC | F:AATGGCAGCTTTGGAGGTGT R:TCTGTTTGGGTGGGAGGTG |
60.9/136 | NM205505 |
| PPARα | F:TGGACGAATGCCAAGGTC R:GATTTCCTGCAGTAAAGGGTG |
60.3/813 | AF163809 |
| CPT-1 | F:CAATGAGGTACTCCCTGAAA R:CATTATTGGTCCACGCCCTC |
57.5/337 | AY675193 |
| ACOX | F:ATGTCACGTTCACCCCATCC R:AGGTAGGAGACCATGCCAGT |
54.0/133 | NM001006205 |
| PPARγ | F:CCAGCGACATCGACCAGTT R:GGTGATTTGTCTGTCGTCTTTCC |
57.5/145 | AF163811 |
| ATGL | F:TCCTTCACCTTCAGCGTCCA R:AGTGTTGTCCTCCATCTGGTC |
54.0/113 | EU852334 |
| LPL | F:CAGTGCAACTTCAACCATACCA R:AACCAGCCAGTCCACAACAA |
60.0/150 | NM205282 |
| GADPH | F:GGTGAAAGTCGGAGTCAACGG R:CGATGAAGGGATCATTGATGGC |
58.4/108 | NM204305 |
1SREBP-1c, sterol regulatory element binding protein-1c; FAS, fatty acid synthase; SCD1, stearoyl-CoA desaturase-1; ACC, acetyl-CoA carboxylase; PPARα, peroxisome proliferator-activated receptor α; CPT-1, carnitine palmitoyltransferase-1; ACOX, acyl-CoA oxidase; PPARγ, peroxisome proliferator-activated receptor γ; ATGL, adipose triglyceride lipase; LPL, lipoprotein lipase; GADPH, glyceraldehyde-3-phosphate dehydrogenase.