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. 2017 Jun 12;4:84. doi: 10.3389/fvets.2017.00084

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Copyright © 2017 Berglund, Fisher, Cameron, Poole and Schnabel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFN-γ stimulation methods. P3 untreated [−/− transforming growth factor-β2 (TGF-β2)], pretreated (+/− TGF-β2), and continuously treated (+/+ TGF-β2) mesenchymal stem cells (MSCs) were stimulated with 1 ng/ml equine IFN-γ over a 72-h period. Untreated and pretreated MSCs not stimulated with IFN-γ were used as controls. Major histocompatibility complex (MHC) I and MHC II expression was measured via FACS following stimulation. Baseline MHC expression was obtained by freezing cells from each treatment group just prior to IFN-γ stimulation.