Figure 5.

Release of cytokines by purified natural killer (NK) cells upon stimulation with selected expansion protocols. Purified NK cells were cultured using different stimulation protocols. On day 6, release of tumor necrosis factor (TNF)-α, IFN-γ, macrophage inflammatory protein (MIP)-1α, monocyte chemoattractant protein (MCP)-1, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and IL-10 were assayed by cytometric bead array analyses. Graphs show mean values and SDs obtained from four independent donors, except GM-CSF, which was measured for three independent donors. Asterisks indicate significant differences (*p < 0.05, one-way ANOVA).