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. 2017 Jun 12;8:671. doi: 10.3389/fimmu.2017.00671

Figure 1.

Figure 1

Bimodal nature of gene expression toward type I and type III interferon (IFN) stimulation. Intestinal epithelial cell lines harboring the bacterial artificial chromosome construct Mx2tRFP were stimulated with different concentrations of IFN-β and IFN-λ3 for 20 h. Expression of the tRFP reporter was measured by flow cytometry (n = 5–7, mean ± SEM). (A) Representative dot plots show Mx2tRFP expression at high and low concentrations of IFN-β and IFN-λ3. (B,C) Percentage of Mx2tRFP-positive cells for all used concentrations of IFN-β and IFN-λ3. P values were calculated by one-way ANOVA. (D,E) Mean fluorescence intensity (MFI) of Mx2tRFP-postive cells. P values were calculated by one-way ANOVA, followed by Tukey’s Multiple Comparison Test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). P values are given for differences among each stimulated group and control group.