Table 1:
Identity of single-species used in this study as determined by Sanger sequencing of 16S rDNA amplicons from different DNA preparations of each species.
| Final sequence | |||
|---|---|---|---|
| Culture a | length (bp) | % | Sequence matches in BlastN organism |
| Escherichia coli | 1440–1696 a | 98 | E. coli numerous strains |
| Microcystis aeruginosa | 1418 | 90 | M. aeruginosa NIES-843 and NIEHS-2549, and M. panniformis FACHB-1757 |
| Pseudomonas fluorescens | 1478–1570 a | 96 | P. fluorescens A506 and LBUM223 |
| Synechococcus elongatus | 1431–1719 a | 99 | S. elongatus PCC 7942, PCC 6301, UTEX 2973 |
a
Multiple DNA preparations from bacterial cultures were used during the progress of the study, and each was tested, yielding for each strain slightly different final 16S sequence lengths, but the same BLAST matches.