. 2017 Feb 24;6(3):1–10. doi: 10.1093/gigascience/gix007

Table 2:

Details of MinION™ WGS output for single-species and synthetic mixtures. Sequencing experiments used the MinION device and new R7.3 flow cells. Libraries were prepared with kit SQK–MAP005 as indicated by (5) and SQK-MAP006 chemistry, indicated by (6). Columns relating to 2D indicate bi-directional reads with quality above Q9.

Experiment	Pores with	Run time	Total bp		Number of 2D	Mean 2D read	MG-RAST	ENA
(chemistry)	reads	(h) ^a	(Mbp)	Total reads	pass reads	length (bp)	accession	accession
Single species
E. coli⁽⁵⁾	430	42	83.6	26 590	1112	5274	4629367.3	ERR1713483
P. fluorescens⁽⁵⁾	453	48	119.4	25 228	777	7784	4629445.3	ERR1713487
M. aeruginosa⁽⁵⁾	377	18	40.8	22 760	569	5676	4629369.3	ERR1713486
S. elongatus⁽⁵⁾	367	23	18.3	6163	224	5101	4629381.3	ERR1713489
Mixtures
Equal ⁽⁵⁾	129	24	26.5	10 592	714	5527	4614572.3	ERR1713484
Equal ⁽⁶⁾	437	44	77.1	12 174	1358	5202	4685746.3	ERR1713485
Rare ⁽⁶⁾	449	18	39.0	6728	899	6194	4685745.3	ERR1713488
Staggered ⁽⁶⁾	300	33	39.0	14 711	3497	2612	4705090.3	ERR1713490

Runs were set to either 24 or 48 h and were allowed to continue until either sufficient sequence data were collected or until the 2D pass rate was greatly reduced.