FIG. 6.

Long-chain acylcarnitines and phosphorylation of acetyl-CoA carboxylase (ACC) after T3 treatment and T3 withdrawal. Adult male mice (8–10 weeks old) were treated with 20 μg of LT3/100 g of body weight for 14 days followed by cessation of LT3 for 10 days. Livers were harvested 14 days (chronic T3) and 24 days after the first LT3 injection (T3 withdrawal). Metabolomics analysis of long-chain acylcarnitines (A) and Western blot analysis for ACC phosphorylation (B) were conducted in the above-described liver samples. After quantification of phosphorylated ACC (pACC) and ACC in each lane, the ratios of intensity of pACC/ACC were expressed as the fold changes relative to the mean value of non-treated mice (0 days). No treatment (blank); acute T3 treatment (black); chronic T3 treatment (hashed); T3 withdrawal (striped) are shown. Data are presented as means ± SEM (n = 3/group). *p < 0.05; **p < 0.01 determined by one-way ANOVA.