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. 2017 Jun 1;23(11-12):535–545. doi: 10.1089/ten.tea.2016.0494

FIG. 3.

FIG. 3.

Effect of time of NANOG expression on the contractile force of MSCs. (A) Schematic illustration of the experimental design. MSCs were cultured in the presence of 1 μg/mL DOX for 1 to 5 days and subsequently seeded in the microdevices for 24 h. (B, C) Western blot and quantification of NANOG and ACTA2 protein levels. (D) Immunostaining of microtissues for F-actin (red) and ACTA2 (green) (scale bar = 200 μm). (E) Contractile force as a function of the time of DOX treatment. * denotes p < 0.05 compared with control; # denotes p < 0.05 compared with 1 and 2 days; and $ denotes p < 0.05 compared with 4 days of NANOG expression. Data are presented as mean ± standard deviation, n = 3 for WB quantifications and n = 20 for force measurement.