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. 2017 Jun 1;23(11-12):535–545. doi: 10.1089/ten.tea.2016.0494

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Copyright 2017, Mary Ann Liebert, Inc.

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FIG. 4. — Additive effects of NANOG and TGF-β1 on restoring the contractile function of senescent microtissues. (A) Effects of NANOG, TGF-β1, or both on the contractile force of microtissues. (B, C) Protein level of ACTA2 in the presence of NANOG, TGF-β1, or both as measured by western blot. (D) Confocal immunofluorescence microscopy of microtissues for ACTA2 (green) protein and F-actin (red) (scale bar = 200 μm). Data are presented as mean ± standard deviation, n = 3 for WB and n = 20 for force measurement. $ denotes p < 0.05 compared with all other samples. TGF-β1, transforming growth factor-beta1.