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Overview of the long single-molecule sequencing protocol. (A) Extract the viral genomic DNA from the whole blood sample. (B) DNA material from the viral mutants is cleaved into sequence fragments using any suitable restriction enzyme. Amplified fragments are sequenced. (C) Long single-molecule reads are mapped to the reference genome. (D) SNVs are detected and assembled into the viral mutant variants. The short read protocol produces equivalent solutions. SNV, single-nucleotide variant.
