FIG. 7.

NBE treatment induces endothelial tumor apoptosis in vivo. (A) There were two tissue sections per group with pixel densitometry done on four separate areas per section using a 20 × magnification objective with a 5 × zoom for each area of analysis. Immunofluorescence detection of cleaved Bax (1:100), caspase 9 (1:100), and cleaved caspase 3 (1:200) proteins in mouse tumor tissue sections collected 10 days after EOMA cell injection. DAPI was used to counterstain the nucleus (scale bar = 100 μm, inset scale bar = 25 μm). Fluorescence intensity analysis showed significant differences in all apoptosis marker proteins in NBE-treated group compared to vehicle treatment. (B) Western blot shows that NBE-treated tumors have significantly lower levels of MRP-1 than vehicle controls *p < 0.05, n = 6. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars