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. 2017 Jun 6;8:15738. doi: 10.1038/ncomms15738

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Blocking chemical synapses of serotonergic Trh493-GAL4 neurons by expressing TNT prevents the amelioration of the depression-like state by sucrose treatment overnight from day 3 (Test 1) to day 4 (Test 2), whereas driverless UAS-TNT controls recover by sucrose solution overnight. (b) Tonic depolarization of serotonergic Trh493-GAL4 neurons by expressing bacterial sodium-ion channels UAS-NaChBac prevents the depression-like state after 3 days vibration treatment; controls lack the driver. Sucrose overnight does not increase the effect of depolarizing Trh493 neurons. After blocking of 5-HT synthesis (α-MTP overnight), neither depolarization of Trh493 neurons nor sucrose treatment can ameliorate the depression-like state. (c–e) Confocal stacks of 10.5 μm thickness, (c) immediately rostral to d. Anti-Fasciclin II staining (FASII, magenta) shows α-/β-lobes and (e) Anti-TRIO labelling the γ-lobes. Trh493-GAL4 driving UAS-mCD8::GFP shows co-localization with α- and γ-lobes, but not with β-lobes. The scale bar denotes 25 μm. (f) Blocking chemical synapses of the mushroom body Kenyon-cells in the mb247-GAL4 pattern (α-/β-/γ-lobes) prohibits the depression-like state even after 4 days of vibration treatment. Controls lack driver or effector. (g) Corresponding experiment to f but with γ-lobe Kenyon-cell driver H24-GAL4; γ-lobes are relevant for bringing about the depression-like state. (h) Blocking of α-/β-lobe Kenyon-cells in the pattern of c739-GAL4 cannot prevent the depression-like state after 3 days of vibration treatment; however, amelioration by sucrose overnight is abolished. Controls lack driver or effector. Black squares, medians; boxes, 25 and 75% quartiles; whiskers, 10 and 90% quantiles; numbers of animals are indicated below each box; NS, not significant; *, P<0.05; **, P<0.01; ***, P<0.001; Kruskal–Wallis test.