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. 2017 Jun 7;8:15699. doi: 10.1038/ncomms15699

Figure 8. Ashen retinal vessels have reduced FLT-1 and excess filopodia.

Figure 8

(a–c) (a) FLT-1 immunofluorescence of retinas from P5 mice with indicated genotypes, scale bars: 50 μm. (b,c) Quantification of FLT-1 in indicated areas via integrated density. Scale bar, 25 μm. (No. of retinas: +/+, n=4; ash/+, n=6; ash/ash, n=4). (d–g) (d) Immunofluorescence of retinas. Quantification of (e) filopodia in the plexus (f) sprouting, and (g) filopodia at the vascular front from P5 mice with indicated genotypes. Red box, magnified at right; arrows, filopodia. Scale bars: 25 μm. (No. of retinas from two independent litters: +/+, n=6, ash/+ n=5; ash/ash, n=7). (h,i) Immunoblot for mFLT-1 in retinal lysates from indicated genotypes at P5 (h) and P8 (i); four replicates per stage. (j) Acyl-RAC and immunoblot for mFLT-1 and Rab27a from retinal lysates at indicated stages; three replicates. (k) Immunoblot of retinal lysates as indicated; three replicates. Statistics: Shown are means +95% CI. One-way ANOVA and pairwise comparison with post-hoc Tukey's range test. *P≤0.05; **P≤0.01; ***P≤0.001; NS, not significant.