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. 2017 Jun 7;8:15472. doi: 10.1038/ncomms15472

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mass-resolved chemical map of P. ultimum hyphae as well as vegetative cells and spores of B. subtilis on top of the silicon wafer. Six negative secondary ion species were detected (a–f) in a 56 × 56 μm sample area. ToF–SIMS images produced were normalized by the total ion counts. The colour scale indicates the relative ion counts for each secondary ion species with warmer colours representing higher relative ion counts and cooler colours representing lower relative ion counts. Arrows in e point to vegetative cells (V). Scale bars, 10 μm.