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. 2005 Feb;71(2):851–857. doi: 10.1128/AEM.71.2.851-857.2005

FIG. 3.

FIG. 3.

Effect of bga1 gene deletion on growth and enzyme formation. (A) Southern analysis of parental strain QM9414 and two bga1 deletion mutants, ΔBGA1-1 and ΔBGA1-12. Genomic DNA was digested with HindIII and probed with a 5-kb HindIII fragment of the bga1 gene. Homologous insertion of the bga1 deletion cassette into the endogenous bga1 locus resulted in an increase (about 500 kb) in the size of the hybridizing band from 5 kb in the parent strain to 5.5 kb in the bga1 deletion mutants. (B) Growth of QM9414 (♦) and strain ΔBGA1 (▵) in shake flasks with peptone (0.1%, wt/vol) and lactose (1%, wt/vol) as carbon sources. The error bars indicate standard deviations for three different experiments. (C) Northern analysis of cbh1 and cbh2 transcript levels during growth on lactose. 18S RNA served as a loading control.