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. 2017 May 28;2017:8932583. doi: 10.1155/2017/8932583

Figure 1.

Figure 1

Sox9, Sox5, and Sox6 gene targeting and their E13.5 transgenic embryos generated for FACS and expression Profiling. (a) Wildtype Sox9 allele (Sox9+) is indicated with exons depicted as black boxes. Translation start site (ATG) as indicated. (b) Sox9 with the F2A-EGFP-FRTNeo inserted in exon 3 (Sox9+(EGFPNeo)) and after FLPe recombination (Sox9+(EGFP)). Sox9+/+(EGFP) E13.5 embryo shown on the right. (c) Sox9-null allele with the EGFP-loxPNeo inserted after the ATG (Sox9−(EGFPNeo)) and after CRE recombination (Sox9−(EGFP)). Sox9-null allele with FRTNeo inserted in exon 1 (Sox9−(Neo)) and after FLPe recombination (Sox9−). Sox9−/−(EGFP) E13.5 chimeric embryo shown on the right. (d) Exon containing the ATG of wildtype Sox5 (Sox5+) shown. EGFP-loxPNeo inserted after the ATG (Sox5−(EGFPNeo)) and after CRE recombination (Sox5−(EGFP)). Sox5+/−(EGFP) E13.5 embryo shown on the right. (e) Exon containing the ATG of wildtype Sox6 (Sox6+) shown. EGFP-loxPNeo inserted after the ATG (Sox6−(EGFPNeo)) and after CRE recombination (Sox6−(EGFP)). Sox6+/−(EGFP) E13.5 embryo shown with a wildtype embryo (WT) under same lighting condition. EGFP, green boxes; F2A, blue ovals; FRT sites, red triangles; loxP sites, yellow triangles (see also Figure S1 and Table S1).