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. 2017 May 28;2017:8932583. doi: 10.1155/2017/8932583

Figure 5.

Figure 5

Genes regulated by the Sox trio. (a) Binding profile of Sox9, Sox5, and Sox6 for Fbxl18, Tgfb2, and Tle3. (b) Transactivation assay for the Sox binding sites found for Fbxl18, Tgfb2, and Tle3. Different Sox proteins were transfected as indicated with the enhancers containing the Sox trio binding sites and the luciferase activity was then measured. (c) Top panel shows the whole mount picture of the 24 hpf zebrafish embryos injected with the Morpholinos for Fbxl18, Tgfb2, and Tle3 along with the wildtype (WT) injected with the scramble Morpholino as a negative control. Bottom panel shows the respective transverse sections of the Morpholino-injected embryos stained with Alcian Blue. The WT embryo shows normal neural tube (NT) and a well-defined emerging sclerotome (black arrow). The morphants at the same stage show extensive midline phenotype with defects in both neural tube and the sclerotome as seen by reduced Alcian Blue staining and loss of structural integrity (see also Figure S5 and Table S6).