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The PIWIL1 was detected by immunohistochemistry. (a and b) PIWIL1 expression in colon cancer tissue. (c and d) PIWIL1 expression in rectum cancer tissue. (e and f) PIWIL1 expression in adjacent tissue. (a, c, and e) Cytoplasm staining of PIWIL1. (b, d, and f) Nucleus staining of PIWIL1.