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The B cells isolated from an alloimmunized woman's blood were activated in vitro by a selected battery of cytokines IL-2/IL-4/IL-6/IL-10/IL-21 (at 1/4/4/2/2 ratio) and 1 μg/ml human CD40 Ab for 7 days, which resulted in an increase in plasma cells (CD19+/CD20−/CD27++/CD38++) from 0.26% on day 0 to 36.25% on day 7 [48].
