Figure 3.

DJC inhibited the activation of JAK2-STAT1/STAT3 pathway in GMCs. (a) Total cell lysates from cells treated with DJC (0.125, 0.5, and 2 mg/ml) or AG490 (20 μM) in the presence of AGEs for 48 h. Proteins were separated by polyacrylamide gels and immunoblotted with anti-phospho-JAK2 (p-JAK2), anti-phospho-STAT1 (p-STAT1), and anti-phospho-STAT3 (p-STAT3) antibodies (upper panels) or antibodies corresponding to the above antibodies (lower panels). This is a representative experiment independently performed three times. (b) Laser densitometry of the gels showed in (a) and two additional phosphorylation experiments. Data are presented as mean ± SD. The columns and error bars represent the mean and SD (n = 3 per group). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 versus Ctrl; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 versus AGEs.