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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Nat Med. 2016 Jul 4;22(8):897–905. doi: 10.1038/nm.4126

Figure 3.

Figure 3

MuSC aging pathways are modulated by fibronectin. (a) Representative western blot analysis for the ERK and p38 MAP kinases from myoblasts grown on collagen I (COL), FN or laminin (LAM) for 72 h. Actin and a Ponceau-stained dominant band at the same molecular weight are shown as loading controls. (b,c) Gene Ontology analysis of statistically differentially regulated genes in microarray data from cells grown on FN versus those grown on Col for 72 h (n = 3 microarrays per condition). Graphs represent counts of up- (b) or downregulated (c) genes that are annotated to the indicated terms. (d) Mapping of proteins and genes that are differentially affected by FN to the KEGG adhesion–signaling system and its associated pathways. Light green indicates factors that are affected by FN at the proteomic level (phosphorylation), red denotes factors whose expression is changed at the transcript level, and blue indicates changes to factors at both levels.