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. Author manuscript; available in PMC: 2018 Apr 21.
Published in final edited form as: ACS Chem Biol. 2017 Mar 9;12(4):928–932. doi: 10.1021/acschembio.7b00159

Figure 2.

Figure 2

A dansylated polymyxin derivative binds LPS on bacterial cell surfaces. (a) Dansyl-PMBN selectively stains Gram-negative organisms. A. baumannii, E. coli, and B. subtilis were incubated with Dansyl-PMBN (12 μM) at room temperature for 30 minutes before being imaged. DIC: Differential interference contrast. Scale bar: 2 μm. (b) Dansyl-PMBN specifically recognizes LPS in liposomes. Phospholipid (PL) liposomes with or without LPS were prepared and incubated with a constant amount of Dansyl-PMBN (1.8 μM). Samples were read on a fluorescent plate reader using Ex/Em=340/520nm (+UV) or without Ex.