Figure 7.

Inhibition of the AKT pathway suppresses MRP1 expression and promotes 5‐FU‐induced apoptosis in BEL/FU cells. (A) LY294002 (10 μm, 20 μm) was used to treat BEL/FU cells, and phosphorylated AKT, AKT, phosphorylated GSK‐3β, and MRP1 were monitored using western blot analysis. (B) AKT/MRP1 pathway expression was monitored by western blot analysis in BEL7402 cells with USP22 overexpression and/or LY294002. (C) Cleaved caspase‐3 and cleaved parp were monitored using western blot analysis in cells treated with LY294002 and/or 5‐FU. Band intensities were semiquantified using image lab 5.0 software and normalized with AKT or β‐actin. Values are represented as the means under the bands.