Figure 1.

Identification of DIMP53‐1 as a potential dual inhibitor of the p53–MDM2/X interactions using yeast. (A) DIMP53‐1 chemical structure. (B) Effect of 0.1–50 μm DIMP53‐1, Nutlin‐3a, and SJ‐172550 on the reestablishment of p53‐induced growth inhibition in MDM2/MDMX‐co‐expressing yeast, for 42 h; results were plotted setting the growth of DMSO‐treated cells expressing p53 alone as 100%; data are mean ± SEM of six independent experiments; values were significantly different from DMSO (***P < 0.001). (C) EC ₅₀ values of DIMP53‐1, Nutlin‐3a, and SJ‐172550 obtained from concentration–response curves presented in (B). (D) Co‐IP was performed with anti‐p53 (IP:p53) or anti‐immunoglobulin G (IgG) antibodies, followed by immunoblotting with anti‐MDM2, anti‐MDMX, and anti‐p53 antibodies in cells treated with 10 and 20 μm of DIMP53‐1 or DMSO for 42 h; whole‐cell lysate (input).