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. 2017 May 12;11(6):640–654. doi: 10.1002/1878-0261.12058

Figure 2.

Figure 2

FEN1 facilitated tumor progression. (A) Generation of cell lines with FEN1 knockdown. (B) Establishment of cell lines with ectopic overexpression of FEN1. In panels A and B, the FEN1 levels in cells were determined by western blotting using anti‐FEN1 antibody. (C) FEN1 knockdown decreased the growth of A549 cells. Data represent mean ± SD from three independent assays. (D) Overexpression of FEN1 promoted the growth of A549 cells. Data represent mean ± SD from three independent assays. (E) Representative image for colony‐forming assay. Panel E showed that FEN1 overexpression enhanced colony formation of A549 cells. (F) A statistic of the clone number for panel E (error bars represent SEM, and statistical significance was determined by paired t‐test. ***P < 0.001. Data were compared between untreated control with respect to treatment, ***P < 0.001). (G). FEN1 knockdown reduced the colony formation efficiency in A549 cells (*P < 0.05). (H). A549 cells with FEN1 overexpression resulted in increased tumor growth and volume compared with parental A549 cells.