TABLE 2.
In silico coverage of dsrAB genes in the GenBank database
| Restriction enzyme (sequence) | No. of dsr genes in GenBanka
|
No. of diagnostic TRFs | Predicted percentagec | Highest TRF frequencyd | ||
|---|---|---|---|---|---|---|
| Cut | No cut | <50 bpb | ||||
| AluI (AG/CT) | 225 | 61 | 95 | 56 | 59.1 | 92 |
| Asp718 (G/GTACC) | 12 | 294 | 75 | 7 | 3.1 | 65 |
| BcnI (CCS/GG) | 136 | 61 | 184 | 37 | 35.7 | 184 |
| DraI (TTT/AAA) | 15 | 354 | 12 | 8 | 3.9 | 12 |
| HaeIII (GG/CC) | 328 | 48 | 5 | 56 | 42.5 | 166 |
| Hsp92II (CATG/) | 360 | 12 | 9 | 61 | 73.2 | 81 |
| NdeII (/GATC) | 247 | 66 | 68 | 70 | 64.8 | 46 |
| RsaI (GT/AC) | 215 | 87 | 79 | 69 | 56.9 | 63 |
| TaqI (T/CGA) | 197 | 126 | 58 | 58 | 51.7 | 56 |
a
Amount out of 381 sequences.
b
TRF smaller than 50 bp.
c
Percentage = 100 × (cut dsr/total dsr).
d
Greatest number of taxons for a particular TRF.