TABLE 1.
Sample layer | Age (103 yr) | Core no.a | Depth (m) | No. of clones
|
|||
---|---|---|---|---|---|---|---|
125 bp | 210 bp | 510 bp | Total | ||||
Modern | 0 | 1 | 0.5 | 25 | 9 | 33 | 67 |
Holocene | 10 | 1 | 4.0 | —b | 21 | 22 | 43 |
Late Pleistocene | 20 | 2 | 4.8 | 34 | 19/25c | 30 | 108 |
Pleistocene | 300-400 | 4 | 9.2 | 25/18c | 35 | 36/22c | 136 |
See reference 27 for core numbers.
Amplification of 125 bp from the 10 ky sample failed. Spiking of samples with Humicula insolens genomic DNA, prior to cycling, revealed inhibition of the PCR in the 10 ky sample (results not shown).
The number after the shill is the number of clones independently obtained in Oxford.