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. 2005 Feb;71(2):1012–1017. doi: 10.1128/AEM.71.2.1012-1017.2005

TABLE 1.

Number of retrieved clones, stratigraphic age, core number, and drilling depth (meters below the surface) for the four sedimentary samples

Sample layer Age (103 yr) Core no.a Depth (m) No. of clones
125 bp 210 bp 510 bp Total
Modern 0 1 0.5 25 9 33 67
Holocene 10 1 4.0 b 21 22 43
Late Pleistocene 20 2 4.8 34 19/25c 30 108
Pleistocene 300-400 4 9.2 25/18c 35 36/22c 136
a

See reference 27 for core numbers.

b

Amplification of 125 bp from the 10 ky sample failed. Spiking of samples with Humicula insolens genomic DNA, prior to cycling, revealed inhibition of the PCR in the 10 ky sample (results not shown).

c

The number after the shill is the number of clones independently obtained in Oxford.