TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Acinetobacter calcoaceticus strain ADP1 | Wild type | ATCC 33305a |
| A. calcoaceticus mutant ΔcphI | Cyanophycinase-negative mutant of ADP1, Kmr | This study |
| Escherichia coli Top10 | F−araD139 Δ(ara, leu)7697 ΔlacX74 galU galK rpsL deoR Φ80dlacZΔM15 endA1 nupG recA1 mdrA Δ(mrr hsdRMS mcrBC) | Invitrogen (San Diego, Calif.) |
| pBluescript SK(−) | Apr, lacPOZ′ | Stratagene (San Diego, Calif.) |
| pKOS3 | Apr, cphI from A. calcoaceticus ADP1 (DSM 587) colinear with lacPO | This study |
a
ATCC, American Type Culture Collection.