TABLE 3.
Effect of variation of the carbon-to-nitrogen ratio on accumulation of cyanophycina
| Sodium glutamate/ammonium sulfate ratio (g/g) | CGP content (% of CDM) |
|---|---|
| 0.5/0.1 | 2.8 ± 0.01 |
| 1.0/0.1 | 2.3 ± 0.2 |
| 1.5/0.1 | 1.7 ± 0.5 |
| 0.5/0.2 | 2.3 ± 0.1 |
| 1.0/0.2 | 2.2 ± 0.04 |
| 1.5/0.2 | 1.8 ± 0.04 |
| 2.0/0.2 | 2.4 ± 0.06 |
| 1.0/0.4 | 1.7 ± 0.05 |
| 1.5/0.4 | 1.9 ± 0.1 |
| 2.0/0.4 | 2.1 ± 0.04 |
a
Cells of A. calcoaceticus strain ADP1 were cultivated for 44 h at 30°C in 250-ml Erlenmeyer flasks without baffles containing 100 ml of Tris-HCl-buffered MSM with different amounts of sodium glutamate and ammonium sulfate. The basic medium was prepared as described in Materials and Methods except for the concentration of the carbon and nitrogen source. At the end of the experiment the cells were harvested by centrifugation, and the cyanophycin content was analyzed. The data are means and standard deviations of three independent experiments.