TABLE 4.

Effects of aspartate, arginine, and glutamate on accumulation of cyanophycin by A. calcoaceticus^a

Substrate(s)b	CGP content (% of CDM)
Aspartate	2.3 ± 0.04
Arginine	14.9 ± 0.16
Aspartate + arginine	4.0 ± 0.18
Arginine + glutamate	2.6 ± 0.10
Aspartate + glutamate	1.2 ± 0.08
Aspartate + argininec	4.5 ± 0.08

^aCells of A. calcoaceticus strain ADP1 were cultivated for 44 h at 30°C in 250-ml Erlenmeyer flasks without baffles containing 100 ml of unbuffered MSM with an initial pH of 7.3 as described for the basic medium in Materials and Methods except that sodium glutamate and ammonium sulfate were omitted as the carbon and nitrogen sources respectively. At the end of the experiment, the cells were harvested by centrifugation, and the cyanophycin content was analyzed. The data are the means and standard deviations of three independent experiments.

^bSodium aspartate (10 mM), arginine (10 mM), and sodium glutamate (10 mM) were added alone or in combination to the medium as carbon and nitrogen sources.

^cThis test was performed in flasks equipped with baffles.