TABLE 5.
Effects of arginine and aspartate on biosynthesis and accumulation of CGPa
| Arginine concn (mM) | Aspartate concn (mM) | CGP content (% of CDM) |
|---|---|---|
| 0 | 10 | 1.6 ± 0.16 |
| 0 | 20 | 1.0 ± 0.05 |
| 15 | 0 | 22.9 ± 0.13 |
| 15 | 10 | 3.9 ± 0.09 |
| 15 | 20 | 3.0 ± 0.11 |
| 30 | 0 | 27.8 ± 0.16 |
| 30 | 10 | 6.8 ± 0.02 |
| 30 | 20 | 3.0 ± 0.54 |
a
Cells of A. calcoaceticus strain ADP1 were cultivated at 30°C in 250-ml Erlenmeyer flasks without baffles containing 100 ml of unbuffered MSM without sodium glutamate and different concentrations of arginine and aspartic acid; the initial pH was 7.3. At the end of the experiment the cells were harvested by centrifugation, and the cyanophycin content was analyzed. The data are means and standard deviations of three independent experiments.