Table II. Primary and secondary Abs for immunohistochemistry.
| Target | Tag | Clone | Host | Isotype | Dilution | Supplier and reference |
|---|---|---|---|---|---|---|
| Primary Abs | ||||||
| CD11b | PE | M1/70 | Rat | IgG2b | 1/200 | BD Biosciences #553311 |
| CD11c | APC | HL3 | Hamster | IgG1 | 1/200 | BD Biosciences #550261 |
| CD31 | ER-MP12 | Rat | IgG2a | 1/1000 | AbD Serotec #MCA2388GA | |
| CD206 | Alexa Fluor 647 | MR5D3 | Rat | IgG2a | 1/100 | AbD Serotec #MCA2235A647 |
| F4/80 | Cl-A3-1 | Rat | IgG2b | 1/100 | AbD Serotec #MCA497 | |
| iNOS | N20 | Rabbit | IgG | 1/100 | Santa Cruz #sc-651 | |
| Ki67 | APC | 16A8 | Rat | IgG2a | 1/100 | Biolegend #652405 |
| Secondary Abs | ||||||
| Anti-rabbit | Alexa Fluor 488 | Goat | 1/200 | Life Technologies #A11008 | ||
| Anti-rat | Alexa Fluor 555 | Goat | 1/1000 | Life Technologies #A21434 |