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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 1;198(11):4513–4523. doi: 10.4049/jimmunol.1601444

Table II. Primary and secondary Abs for immunohistochemistry.

Target Tag Clone Host Isotype Dilution Supplier and reference
Primary Abs
 CD11b PE M1/70 Rat IgG2b 1/200 BD Biosciences #553311
 CD11c APC HL3 Hamster IgG1 1/200 BD Biosciences #550261
 CD31 ER-MP12 Rat IgG2a 1/1000 AbD Serotec #MCA2388GA
 CD206 Alexa Fluor 647 MR5D3 Rat IgG2a 1/100 AbD Serotec #MCA2235A647
 F4/80 Cl-A3-1 Rat IgG2b 1/100 AbD Serotec #MCA497
 iNOS N20 Rabbit IgG 1/100 Santa Cruz #sc-651
 Ki67 APC 16A8 Rat IgG2a 1/100 Biolegend #652405
Secondary Abs
 Anti-rabbit Alexa Fluor 488 Goat 1/200 Life Technologies #A11008
 Anti-rat Alexa Fluor 555 Goat 1/1000 Life Technologies #A21434