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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Plasmid. 2014 Oct 24;76:87–94. doi: 10.1016/j.plasmid.2014.10.004

Fig. 1.

Fig. 1

Schematic of pXB300 construction. (A.) The steps in the construction of pXB300 are indicated. p11AAZDYP was digested with PacI, treated with the Klenow fragment of DNA polymerase to blunt the ends, then digested with SalI. In a separate reaction, pBAD18 was digested with ClaI, treated with the Klenow fragment of DNA polymerase to blunt the ends, and then digested with SalI. The resulting 1.4 Kb fragment from p11AAZDYP was then ligated with the 3.3 kb pBAD18 fragment to generate pXB300. (B.) The pXB300 multiple cloning site region showing unique restrictions sites, the tetA promoter including the -35 and -10 promoter elements, and the TetR operator sequences (O1 and O2).