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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Plasmid. 2014 Oct 24;76:87–94. doi: 10.1016/j.plasmid.2014.10.004

Fig. 4.

Fig. 4

Complementation of V. cholerae virulence mutants for cholera toxin production. The V. cholerae ToxR regulon mutant strains were grown overnight under AKI conditions in AKI broth supplemented with the indicated concentrations of aTc. The culture supernatants were then used in the cholera toxin (CT) ELISA to quantify CT production as described in the methods. (A) V. cholerae strain XBV153 (ΔaphA) containing pEW1 (pXB300-aphA). (B) V. cholerae strain JB460 (ΔtoxT) containing pXB320 (pXB300-toxT). The results are representative of three experiments ± SD.