REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli K-12 MG1655/wild type	Coli Genetic Stock Center	MG1655
E. coli/ΔmlaE ΔpqiA ΔyebS mutant	This work	sBE120
E. coli/ΔmlaE mutant	This work	sBE104
E. coli/ΔmlaD mutant	This work	sBE113
E. coli K-12 BW25113/wild type	This work	N/A
Rosetta2 DE3	Novagen	71400
E. coli K-12 BW25113 ΔmlaD ΔpqiAB ΔyebST	This work	N/A
Chemicals, Peptides, and Recombinant Proteins
IPTG	Sigma	I6758
imidazole	Sigma	I5513
EDTA	Sigma	ED2SS
chloroform	Alfa Aesar	22920
methanol	Fisher	M/4060/PB17
phosphomolybdic acid	Acros organics	417895000
carbonyl cyanide-m-chlorophenyl hydrazine	Sigma	C2759
NiNTA resin	Qiagen	30210
JCSG core I screen	Qiagen	130924
JCSG core II screen	Qiagen	130925
JCSG core III screen	Qiagen	130926
JCSG core IV screen	Qiagen	130927
DDM	Anatrace	D310S
Uranyl formate	Electron microscopy sciences	22451
DAPI	molecular probes	D1306
Propidium Iodide	molecular probes	P3566
amphipol A8-35	Anatrace	A835
PE standard	Avanti Lipids	840027
PG standard	Avanti Lipids	841188
CL standard	Avanti Lipids	841199
ethylene glycol	Hampton Research	HR2-621
Seleno-methionine	Chem-impex international	662
Bio-beads	Biorad	1523920
Deposited Data
Crystal structure of MlaD core MCE domain	PDB	5UW8
Crystal structure of MlaD periplasmic domain	PDB	5UW2
Crystal structure of MlaC	PDB	5UWA
Re-refined coordinates for 4FCZ	PDB	5UWB
Cryo EM reconstruction for PqiB periplasmic domain	EMDB	EMD-8606
Cryo EM structure for PqiB periplasmic domain	PDB	5UVN
Cryo EM reconstruction for MlaFEDB complex	EMDB	EMD-8610
Negative stain EM reconstruction for PqiB	EMDB	EMD-8612
Negative stain EM reconstruction for YebT	EMDB	EMD-8611
Recombinant DNA
pET22b(+)-mlaD(Δaa1-28) (MlaD for TLC)	This work	N/A
pET22b(+)-pqiB(Δaa1-40) (PqiB for TLC)	This work	N/A
pET22b(+)-yebT(Δaa1-40) (YebT for TLC)	This work	N/A
Custom pET vector with N-terminal 6xHis tag	This work	pBE1092
Arabinose inducible expression vector	Invitrogen	pBAD-His
Custom pET vector with C-terminal 6xHis tag	This work	pDCE587
Expression construct for MlaD(32-183)	This work	pBE1160
Expression construct for MlaD(32-140)	This work	pBE1161
Complementation construct containing MlaFEDCB operon, MlaD mutant L106N/L107N	This work	pBE1326
Complementation construct containing MlaFEDCB operon, MlaD mutant L106N/L107N + 2 residue deletion in B5-6	This work	pBE1327
Complementation construct containing MlaFEDCB operon, MlaD mutant L106N/L107N + 4 residue deletion in B5-6	This work	pBE1328
Complementation construct containing MlaFEDCB operon, MlaD mutant C-terminal deletion 141–183	This work	pBE1330
Complementation construct containing MlaFEDCB operon, MlaD mutant F150	This work	pBE1329
Expression construct for MlaFEDCB, His-tagged MlaD	This work	pBE1200
Expression construct for MlaC	This work	pBE1203
Expression construct for MlaA	This work	pBE1242
YebT negative stain EM reconstruction	This work	pBE1287
Expression construct for PqiB	This work	pBE1163
Expression construct for PqiB, C-terminally truncated	This work	pBE1222
Expression construct for MlaD(32-140), Leu42Met mutant	This work	pBE1168
Expression construct for MlaD(32-140), Leu52Met mutant	This work	pBE1169
Expression construct for MlaD(32-140), Leu79Met mutant	This work	pBE1171
Expression construct for MlaD(32-140), Leu84Met mutant	This work	pBE1172
Expression construct for MlaD(32-140), Leu99Met mutant	This work	pBE1173
Expression construct for MlaD(32-140), Leu106Met mutant	This work	pBE1174
Expression construct for MlaD(32-140), Leu107Met mutant	This work	pBE1175
Expression construct for MlaD(32-140), Leu112Met mutant	This work	pBE1176
Expression construct for MlaD(32-140), Leu114Met mutant	This work	pBE1177
Expression construct for MlaD(32-140), Leu123Met mutant	This work	pBE1178
Expression construct for MlaD(32-140), Leu128Met mutant	This work	pBE1179
Software and Algorithms
XDS Package (including XDS, XSCALE, and XDSCONV)	(Kabsch, 2010)	http://xds.mpimf-heidelberg.mpg.de
Phaser	(McCoy et al, 2007)	http://www-struct-med.cimr.cam.ac.uk/phaser_obsolete/
SHELX Package (including SHELX C, D, and E)	(Sheldrick, 2010)	http://shelx.uni-ac.gwdg.de/SHELX/
Phenix Package (including phenix.refine, phenix.real_space_refine, phenix.autobuild, phenix.reduce, phenix.reflection_file_converter, phenix.mr_rosetta)	(Adams et al, 2010)	https://www.phenix-online.org
Coot	(Emsley, et al, 2010)	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Chimera	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/
PyMOL	N/A	https://www.pymol.org/
HHblits	(Remmert et al., 2012)	https://toolkit.tuebingen.mpg.de/hhblits
Phyre2	(Kelley et al., 2015)	www.sbg.bio.ic.ac.uk/phyre2
Simple 2.0	(Elmlund and Elmlund, 2012; Elmlund et al., 2013)	http://www.simplecryoem.com/2.0/
SAMUEL	N/A	http://liao.hms.harvard.edu/samuel
Motioncor2	Zheng et al, 2016	http://msg.ucsf.edu/em/software/motioncor2.html
Gctf	Zhang, 2016	http://www.mrc-lmb.cam.ac.uk/kzhang/
Gautomatch	N/A	http://www.mrc-lmb.cam.ac.uk/kzhang/
Relion 1.4	Scheres, 2012a	https://www2.mrc-lmb.cam.ac.uk/relion/index.php/Download_%26_install
UCSF Tomography	N/A	http://www.msg.ucsf.edu/Tomography/tomography_main.html
SerialEM	Mastronarde, 2005	http://bio3d.colorado.edu/SerialEM/
GREMLIN	Ovchinnikov et al., 2014	http://gremlin.bakerlab.org
Other
Quantifoil grids 2/2	Quantifoil	N/A
Quantifoil grids 1.2/1.3, 400 mesh	Quantifoil	N/A
TLC plates	Merck Millipore	105554
microcapillary tubes	Sigma Aldrich	Z611239
SA biosensors for octet	Pall	18-5019