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Viable numbers of staphylococci and salmonellae in horizontal cross sections of a dual-species biofilm grown at 25°C for 12 days. Data points represent mean values of three independent experiments with standard deviation. The experimental limit of detection was 2 log CFU/20-μm section. Statistical analysis (t test) revealed significant differences (P > 0.05) between the staphylococcal and salmonellae populations throughout the biofilm.
