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. 2017 May 24;51(1):169–183. doi: 10.3892/ijo.2017.4018

Figure 2.

Figure 2

Activity of Fyn regulates the phosphorylation and nuclear localization of hnRNP E1 via PAK1. (A–C) Western blot analysis revealed the relative expression levels of hnRNP E1 and PAK1 protein among BxPC3, BxPC3GFP and BxPC3KdFyn cells. β-actin blotting was used as a loading control. (D and E) Co-immunoprecipitation analysis showed the threonine-phosphorylation of hnRNP E1 and the interaction of hnRNP E1 with PAK1 among BxPC3, BxPC3GFP and BxPC3KdFyn cells. (F and G) Confocal microscopy analysis showed that the nuclear distribution of hnRNP E1 was increased in BxPC3KdFyn cells compared with BxPC3GFP cells. *P<0.05. Data are presented as the mean ± standard deviation from ≥3 independent experiments.