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. 2017 May 24;51(1):169–183. doi: 10.3892/ijo.2017.4018

Figure 3.

Figure 3

Fyn regulates the alternative splicing of integrin β1 via hnRNP E1. (A) Co-immunoprecipitation analysis showed the expression of GFP-hnRNP E1 fusion protein in BxPC3 cells. (B, F and G) Splicing of integrin β1 was analyzed under different Fyn activity levels or hnRNP E1 expression levels in BxPC3 cells. (C–E) Knockdown of hnRNP E1 expression decreased the phosphorylation of hnRNP E1 in BxPC3 cells. (H and I) Splicing of integrin β1 was analyzed under different Fyn activity levels or hnRNP E1 expression levels in Panc1 cells. (J) RNA immunoprecipitation showed the interaction between hnRNP E1 and the pre-mRNA of integrin β1. *P<0.05. Data are presented as the mean ± standard deviation from ≥3 independent experiments. Sequences of the PCR primers for integrin β1 pre-mRNA, which cover the exon and intron sequences of integrin β1C (Fig. 4A) are listed in Table I.