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. 2017 May 24;51(1):128–144. doi: 10.3892/ijo.2017.4019

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Copyright: © Abeni et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Evaluation of mRNA expression by RT-qPCR in sorafenib treated and untreated HCC cells. The mRNA expression of the genes BIRC3, FOXO3, MAPK3, SMAD2 and TSC2 was evaluated in HA22T/VGH (A) and SKHep1C3 (B) sorafenib-treated and untreated cells by RT-qPCR, to establish the relationship between DNA methylation changes and gene expression. Relative expression was calculated using ΔΔCt method and an untreated cell sample as a reference. The housekeeping gene YWHAZ was used. Error bars of the RQ panels refer to standard deviation of the RQ means of the two experiment performed. ^*P<0.05, ^**P<0.01 and ^***P<0.001 indicate that the expression difference between the two cellular conditions was statistically significant.