Table I.
Gene name | Forward primer sequence 5′-3′ | Reverse primer sequence 5′-3′ | Genebank accession no. |
---|---|---|---|
CCL2 | CAGCCAGATGCAATCAATGCC | TGGAATCCTGAACCCACTTCT | NM_002982.3 |
CCL17 | CTTCTCTGCAGCACATCCAC | AGTACTCCAGGCAGCACTCC | NM_002987.2 |
CCL18 | CTCCTTGTCCTCGTCTGCAC | TCAGGCATTCAGCTTCAGGT | NM_002988.3 |
CXCL9 | CCAGTAGTGAGAAAGGGTCGC | AGGGCTTGGGGCAAATTGTT | NM_002416.2 |
ERα | GGGAAGTATGGCTATGGAATCTG | TGGCTGGACACATATAGTCGTT | NM_000125.3 |
ERβ1 | TCCATCGCCAGTTATCACATCT | CTGGACCAGTAACAGGGCTG | NM_001437.2 |
GAPDH | CAACAGCGACACCCACTCCT | CACCCTGTTGCTGTAGCCAAA | NM_002046.4 |
HER-2 | TGTGACTGCCTGTCCCTACAA | CCAGACCATAGCACACTCGG | NM_001005862.2 |
IL6 | GATCCAAAAACCACCCCTGACCC | CAATCTGAGGTGCCCATGCTAC | NM_000600.3 |
IL8 | CATGACTTCCAAGCTGGCCGTG | CCACTCTCAATCACTCTCAGTTC | NM_000584.3 |
IL10 | CTGGGGGAGAACCTGAAGA | GGCCTTGCTCTTGTTTTCAC | NM_000572.2 |
IL12 | CAGCCTGGGAAACATAACAAGAC | CTCCTGCCTCATCCTCCTGAA | NM_002187.2 |
MMP-9 | GGGACGCAGACATCGTCATC | TCGTCATCGTCGAAATGGGC | NM_004994.2 |
NFκB | CCAACAGATGGCCCATACCT | AACCTTTGCTGGTCCCACAT | NM_001165412.1 |
p21 | TTAGCAGCGGAACAAGGAGT | AGCCGAGAGAAAACAGTCCA | NM_000389.4 |
P27 | TAATTGGGGCTCCGGCTAACT | TGCAGGTCGCTTCCTTATTCC | NM_004064.3 |
POLR2F | ATGTCAGACAACGAGGACAATTT | TTCGGCATTCTCCAAGTCATC | NM_001301129.1 |
PR | ACCCGCCCTATCTCAACTACC | AGGACACCATAATGACAGCCT | NM_000926.4 |
TNF-α | CCTCTCTCTAATCAGCCCTCTG | GAGGACCTGGGAGTAGATGAG | NM_000594.3 |
uPAR | GAGCTATCGGACTGGCTTGAA | CGGCTTCGGGAATAGGTGAC | NM_002659.3 |
qPCR, quantitative PCR; CCL, chemokine (C-C motif) ligand; CXCL, chemokine (C-X-C motif) ligand; ERα, estrogen receptor α; ERβ1, estrogen receptor β; GAPDH, glyceraldehyde 3 phosphate dehydrogenase; HER-2, human epidermal growth factor receptor 2; IL, interleukin; MMP, matrix metalloproteinase; NF, nuclear factor; POLR2F, RNA polymerase II subunit F; PR, progesterone receptor; TNF, tumor necrosis factor; uPAR, urokinase plasminogen activator receptor.