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. 2017 May 11;51(1):104–114. doi: 10.3892/ijo.2017.3996

Table I.

Primer sequences used for qPCR.

Gene name Forward primer sequence 5′-3′ Reverse primer sequence 5′-3′ Genebank accession no.
CCL2 CAGCCAGATGCAATCAATGCC TGGAATCCTGAACCCACTTCT NM_002982.3
CCL17 CTTCTCTGCAGCACATCCAC AGTACTCCAGGCAGCACTCC NM_002987.2
CCL18 CTCCTTGTCCTCGTCTGCAC TCAGGCATTCAGCTTCAGGT NM_002988.3
CXCL9 CCAGTAGTGAGAAAGGGTCGC AGGGCTTGGGGCAAATTGTT NM_002416.2
ERα GGGAAGTATGGCTATGGAATCTG TGGCTGGACACATATAGTCGTT NM_000125.3
ERβ1 TCCATCGCCAGTTATCACATCT CTGGACCAGTAACAGGGCTG NM_001437.2
GAPDH CAACAGCGACACCCACTCCT CACCCTGTTGCTGTAGCCAAA NM_002046.4
HER-2 TGTGACTGCCTGTCCCTACAA CCAGACCATAGCACACTCGG NM_001005862.2
IL6 GATCCAAAAACCACCCCTGACCC CAATCTGAGGTGCCCATGCTAC NM_000600.3
IL8 CATGACTTCCAAGCTGGCCGTG CCACTCTCAATCACTCTCAGTTC NM_000584.3
IL10 CTGGGGGAGAACCTGAAGA GGCCTTGCTCTTGTTTTCAC NM_000572.2
IL12 CAGCCTGGGAAACATAACAAGAC CTCCTGCCTCATCCTCCTGAA NM_002187.2
MMP-9 GGGACGCAGACATCGTCATC TCGTCATCGTCGAAATGGGC NM_004994.2
NFκB CCAACAGATGGCCCATACCT AACCTTTGCTGGTCCCACAT NM_001165412.1
p21 TTAGCAGCGGAACAAGGAGT AGCCGAGAGAAAACAGTCCA NM_000389.4
P27 TAATTGGGGCTCCGGCTAACT TGCAGGTCGCTTCCTTATTCC NM_004064.3
POLR2F ATGTCAGACAACGAGGACAATTT TTCGGCATTCTCCAAGTCATC NM_001301129.1
PR ACCCGCCCTATCTCAACTACC AGGACACCATAATGACAGCCT NM_000926.4
TNF-α CCTCTCTCTAATCAGCCCTCTG GAGGACCTGGGAGTAGATGAG NM_000594.3
uPAR GAGCTATCGGACTGGCTTGAA CGGCTTCGGGAATAGGTGAC NM_002659.3

qPCR, quantitative PCR; CCL, chemokine (C-C motif) ligand; CXCL, chemokine (C-X-C motif) ligand; ERα, estrogen receptor α; ERβ1, estrogen receptor β; GAPDH, glyceraldehyde 3 phosphate dehydrogenase; HER-2, human epidermal growth factor receptor 2; IL, interleukin; MMP, matrix metalloproteinase; NF, nuclear factor; POLR2F, RNA polymerase II subunit F; PR, progesterone receptor; TNF, tumor necrosis factor; uPAR, urokinase plasminogen activator receptor.