Fig 5. The effects of brain-derived neurotrophic factor (BDNF) and TrkB on the migratory activity of the MDA-MB-231 line.

The cell morphology is presented (A) and the protein expression of BDNF and TrkB (B) were validated in the parent, the △BDNF cell line and the △TrkB cell line. MDA-MB-231 parent cells or the △BDNF and the △TrkB cell lines (2×10⁴) were seeded into a 3.5 cm Petri dish containing an insert for migration assay (C). The migratory area was quantified (D). Data were expressed as mean ± SEM. *, p< 0.05 compared to vehicle group; #, p< 0.01 compared to positive control group by Repeatedly measured one way ANOVA, n = 5).