Table 1. Forward and reverse primers for PCR amplification of the twelve selected positional candidate genes located within 1Mb of the genome-wide significant markers for EBVs FCR and D110.
| Gene | Ensembl reference | Size (bp) | Forward | Reverse |
|---|---|---|---|---|
| CD164 | ENSSSCG00000004414 | 713 | TGTGTCTGTCCAGTTTCTTCGC | TGAAGTCAGGCTGGGGATTACG |
| NR2E1 | ENSSSCG00000004384 | 706 | TCTCCCTTCCCTCTCTTCACCT | ACCTACGCTGCCCTCTGATTTC |
| SMPD2 | ENSSSCG00000004408 | 697 | CCTCCTCTCTGACCCTCTCTCT | TGGGGCTGTCTGTTTCTTCC |
| PRKDC | ENSSSCG00000006274 | 735 | AGGAAACACGCCTCAGTTGGTA | ACGCAGGAGACAGAAGGAAAGC |
| SELL | ENSSSCG00000006287 | 706 | TCTCAAAACAAATGTCTGTGGCTGT | GGTTATCTTCTGGGCAACTCACC |
| SELP | ENSSSCG00000006288 | 350 | ACCTGAATCCAACCTCTCTCCA | TGCATCTGAAGTAGCAAGTCGT |
| OPRD1 | ENSSSCG00000027401 | 718 | GCTCCCATCCACATCTTCGTCA | CCCCTCAATTCCACCTTCCTCA |
| WDTC1 | ENSSSCG00000003570 | 567 | CCAGGGACCAAGACAACCGA | CACCATACCTCACAGCAACGC |
| AKR1C3 | ENSSSCG00000030447 | 792 | GCTGACACTTAGCAGTTGAGGAATA | GGTGGAGGAAAGAGGAGTTAAATACA |
| KLF6 | ENSSSCG00000028828 | 702 | GACCAACAGCCTGAACTCGGA | CCCTGAGTCTCACTTCCCCAAA |
| MBD5 | ENSSSCG00000015667 | 773 | ACTTGGAAGCCCTGATGTTTTCAC | ACCCTATCGTTGACCTTGGTGAC |
| MMADHC | ENSSSCG00000028646 | 696 | GGATTCTCCGTTGATGATCTTGGC | CCTTATTCTTCTTTCCCGCACAAAC |