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. 2005 Feb;71(2):1058–1065. doi: 10.1128/AEM.71.2.1058-1065.2005

FIG. 4.

FIG. 4.

Spatial arrangement of Bacteria, Archaea, and D. hafniense in the anaerobic FF reactor. Biofilm samples were fixed, permeabilized, hybridized with fluorescently labeled oligonucleotides, and then counterstained with YOYO-1 before being examined by CLSM. FISH was performed with EUB338-Cy3 (Bacteria, panel A), ARC915-Cy3 (Archaea, panel B), and PCP-1-4-Cy3 and PCP-1-8-Cy5 (D. hafniense, panel C). Panels A, B, and C are biofilms taken after 56, 116, and 225 days of operation, respectively. Bars correspond to 20 μm.